In this interview, David Zierler, Oral Historian for AIP, Interviews Robert Best, Senior Investigator, Laboratory of Chemical Physics, NIDDK, at the NIH. Best recounts his childhood in South Africa, his education in math and physics at the University of Cape Town, and his graduate work at Cambridge where he worked with Jane Clarke on protein folding. Best describes his post-doctoral research that led to his connection with Bill Eaton and Gerhard Hummer, and his decision to join the NIH. In the last third of the interview, Best explains his recent work on the single molecule fluorescence, DNA binding proteins, and novel simulation methodology.